Acacia

Leaves contain flavonoids, terpenoids, and tannins and have abortifacient properties

Stem bark contains 3-(Z)-trans coumaroylbetulin and 3-(E)-cis coumaroylbetulin and has antimalarial properties

Stem bark contains betulinic acid and betulinic acid-3-trans-caffeate and has antimicrobial and antioxidant properties

Leaves contain diterpenes and tannins and have antimicrobial and antioxidant properties

Leaves contain myricetin-3-O-α-l-rhamnoside and quercetin-3-O-α-l-rhamnoside and have antioxidant and cytotoxic properties

Leaves contain β-sitosterol and epigallocatechin and have antilisterial properties

Berlin:
Robinia pseudoacacia

• toxalbumin robin
• glucoside robinin

Extraction: There are different methods of extraction, such as maceration, percolation, and soxhlet extraction. The choice of method depends on the type of plant material and the desired compound(s) to be extracted. For example, alkaloids can be extracted using acid-base extraction methods.

Fractionation: After extraction, the crude extract can be fractionated to isolate the desired compound(s). Fractionation can be done using various techniques such as chromatography, distillation, and crystallization.
Characterization: Once the compound(s) have been isolated, they can be characterized using various analytical techniques such as spectroscopy and chromatography.